MAP-XS
 Methyl Assignment Prediction from Xray Structures

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More information on the group

PDB File

Your PDB file must contain Hydrogens. E.g.
ATOM      1  N   ILE    12      -3.275   3.609 -10.344  1.00 57.84
ATOM      2  H   ILE    12      -2.575   2.896 -10.197  1.00  0.00
ATOM      3  CA  ILE    12      -3.008   5.004  -9.861  1.00 57.61
ATOM      4  HA  ILE    12      -3.019   5.682 -10.714  1.00  0.00

Sequence File

E.g.
THR 13
VAL 14
PHE 15
SER 16

XEASY .prot file

E.g.
   1 999.000 0.000 N      13
   2 999.000 0.000 HN     13
   3 999.000 0.000 CA     13
   4 999.000 0.000 HA     13
   5 999.000 0.000 CB     13
   6 999.000 0.000 HB     13
   7 999.000 0.000 QG2    13
   8 999.000 0.000 HG1    13
   9 999.000 0.000 CG2    13
  10 999.000 0.000 HG21   13
  11 999.000 0.000 HG22   13
  12 999.000 0.000 HG23   13
  13 999.000 0.000 C      13
  14 999.000 0.000 N      14
  15 999.000 0.000 HN     14
  16 999.000 0.000 CA     14
  17 999.000 0.000 HA     14
  18 999.000 0.000 CB     14
  19 999.000 0.000 HB     14
  20   1.277 0.000 QG1    14
  21 999.000 0.000 QG2    14
  22  17.770 0.005 CG1    14
  23 999.000 0.000 HG11   14

XEASY .peaks file

E.g.
# Number of dimensions 3
#INAME 1 C13 (bonded with the H1)
#INAME 2 C13
#INAME 3 H1
   2  26.077  26.077   1.604 2 U          1.646e+03  0.00e+00 m   0 1163 1163 1158 0
   3  25.759  25.759   1.597 2 U          1.012e+03  0.00e+00 m   0    0    0    0 0
   4  23.131  23.131   1.590 2 U          6.725e+03  0.00e+00 m   0 3613 3613 3608 0
   5  21.936  21.936   1.583 2 U          3.486e+04  0.00e+00 m   0 1177 1177 1175 0

Other formats

Other formats are not currently supported using the web tool. You may prefer to run MAP-XS locally. Please visit the download page for more details.


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Please cite: Automated Assignment in Selectively Methyl-Labeled Proteins
Xu Y, Liu M, Simpson PJ, Isaacson R, Cota E, Marchant J, Yang D, Zhang X, Freemont P, Matthews S.
J Am Chem Soc. 131:9480-1.



© Matthews group, Imperial College, London